PoplarV1, Main, Exploration, bibRecord, 001E95

Antisense expression of the fasciclin-like arabinogalactan protein FLA6 gene in Populus inhibits expression of its homologous genes and alters stem biomechanics and cell wall composition in transgenic trees.

Identifieur interne : 001E95 ( Main/Exploration ); précédent : 001E94; suivant : 001E96

Antisense expression of the fasciclin-like arabinogalactan protein FLA6 gene in Populus inhibits expression of its homologous genes and alters stem biomechanics and cell wall composition in transgenic trees.

Auteurs : Haihai Wang [République populaire de Chine] ; Chunmei Jiang [République populaire de Chine] ; Cuiting Wang [République populaire de Chine] ; Yang Yang [République populaire de Chine] ; Lei Yang [République populaire de Chine] ; Xiaoyan Gao [République populaire de Chine] ; Hongxia Zhang [République populaire de Chine]

Source :

Journal of experimental botany [ 1460-2431 ] ; 2015.

RBID : pubmed:25428999

Descripteurs français

English descriptors

KwdEn :
- Biomechanical Phenomena (MeSH), Cell Wall (chemistry), Cell Wall (genetics), Cell Wall (metabolism), DNA, Antisense (genetics), DNA, Antisense (metabolism), Gene Expression (MeSH), Gene Expression Regulation, Plant (MeSH), Mucoproteins (genetics), Mucoproteins (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Stems (chemistry), Plant Stems (genetics), Plant Stems (metabolism), Plants, Genetically Modified (chemistry), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (chemistry), Populus (genetics), Populus (metabolism), Xylem (chemistry), Xylem (genetics), Xylem (metabolism).
MESH :
- chemical , genetics : DNA, Antisense, Mucoproteins, Plant Proteins.
- chemistry : Cell Wall, Plant Stems, Plants, Genetically Modified, Populus, Xylem.
- genetics : Cell Wall, Plant Stems, Plants, Genetically Modified, Populus, Xylem.
- metabolism : Cell Wall, DNA, Antisense, Mucoproteins, Plant Proteins, Plant Stems, Plants, Genetically Modified, Populus, Xylem.
- Biomechanical Phenomena, Gene Expression, Gene Expression Regulation, Plant.

Abstract

Fasciclin-like arabinogalactan proteins (FLAs) play important roles in the growth and development of roots, stems, and seeds in Arabidopsis. However, their biological functions in woody plants are largely unknown. In this work, we investigated the possible function of PtFLA6 in poplar. Quantitative real-time PCR, PtFLA6-yellow fluorescent protein (YFP) fusion protein subcellular localization, Western blotting, and immunohistochemical analyses demonstrated that the PtFLA6 gene was expressed specifically in the xylem of mature stem, and PtFLA6 protein was distributed ubiquitous in plant cells and accumulated predominantly in stem xylem fibres. Antisense expression of PtFLA6 in the aspen hybrid clone Poplar davidiana×Poplar bolleana reduced the transcripts of PtFLA6 and its homologous genes. Transgenic plants that showed a significant reduction in the transcripts of PtFLAs accumulated fewer PtFLA6 and arabinogalactan proteins than did the non-transgenic plants, leading to reduced stem flexural strength and stiffness. Further studies revealed that the altered stem biomechanics of transgenic plants could be attributed to the decreased cellulose and lignin composition in the xylem. In addition expression of some xylem-specific genes involved in cell wall biosynthesis was downregulated in these transgenic plants. All these results suggest that engineering the expression of PtFLA6 and its homologues could modulate stem mechanical properties by affecting cell wall composition in trees.

DOI: 10.1093/jxb/eru479
PubMed: 25428999
PubMed Central: PMC4339592

Affiliations:

République populaire de Chine

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Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en">Antisense expression of the fasciclin-like arabinogalactan protein FLA6 gene in Populus inhibits expression of its homologous genes and alters stem biomechanics and cell wall composition in transgenic trees.</title>
<author><name sortKey="Wang, Haihai" sort="Wang, Haihai" uniqKey="Wang H" first="Haihai" last="Wang">Haihai Wang</name>
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<author><name sortKey="Jiang, Chunmei" sort="Jiang, Chunmei" uniqKey="Jiang C" first="Chunmei" last="Jiang">Chunmei Jiang</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032</wicri:regionArea>
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<wicri:noRegion>Shanghai 200032</wicri:noRegion>
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<author><name sortKey="Yang, Yang" sort="Yang, Yang" uniqKey="Yang Y" first="Yang" last="Yang">Yang Yang</name>
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<series><title level="j">Journal of experimental botany</title>
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<imprint><date when="2015" type="published">2015</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomechanical Phenomena (MeSH)</term>
<term>Cell Wall (chemistry)</term>
<term>Cell Wall (genetics)</term>
<term>Cell Wall (metabolism)</term>
<term>DNA, Antisense (genetics)</term>
<term>DNA, Antisense (metabolism)</term>
<term>Gene Expression (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Mucoproteins (genetics)</term>
<term>Mucoproteins (metabolism)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plant Stems (chemistry)</term>
<term>Plant Stems (genetics)</term>
<term>Plant Stems (metabolism)</term>
<term>Plants, Genetically Modified (chemistry)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (metabolism)</term>
<term>Populus (chemistry)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
<term>Xylem (chemistry)</term>
<term>Xylem (genetics)</term>
<term>Xylem (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN antisens (génétique)</term>
<term>ADN antisens (métabolisme)</term>
<term>Expression des gènes (MeSH)</term>
<term>Mucoprotéines (génétique)</term>
<term>Mucoprotéines (métabolisme)</term>
<term>Paroi cellulaire (composition chimique)</term>
<term>Paroi cellulaire (génétique)</term>
<term>Paroi cellulaire (métabolisme)</term>
<term>Phénomènes biomécaniques (MeSH)</term>
<term>Populus (composition chimique)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Tiges de plante (composition chimique)</term>
<term>Tiges de plante (génétique)</term>
<term>Tiges de plante (métabolisme)</term>
<term>Végétaux génétiquement modifiés (composition chimique)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
<term>Xylème (composition chimique)</term>
<term>Xylème (génétique)</term>
<term>Xylème (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Antisense</term>
<term>Mucoproteins</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term>
<term>Plant Stems</term>
<term>Plants, Genetically Modified</term>
<term>Populus</term>
<term>Xylem</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Paroi cellulaire</term>
<term>Populus</term>
<term>Tiges de plante</term>
<term>Végétaux génétiquement modifiés</term>
<term>Xylème</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Wall</term>
<term>Plant Stems</term>
<term>Plants, Genetically Modified</term>
<term>Populus</term>
<term>Xylem</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN antisens</term>
<term>Mucoprotéines</term>
<term>Paroi cellulaire</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Tiges de plante</term>
<term>Végétaux génétiquement modifiés</term>
<term>Xylème</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term>
<term>DNA, Antisense</term>
<term>Mucoproteins</term>
<term>Plant Proteins</term>
<term>Plant Stems</term>
<term>Plants, Genetically Modified</term>
<term>Populus</term>
<term>Xylem</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN antisens</term>
<term>Mucoprotéines</term>
<term>Paroi cellulaire</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Tiges de plante</term>
<term>Végétaux génétiquement modifiés</term>
<term>Xylème</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Biomechanical Phenomena</term>
<term>Gene Expression</term>
<term>Gene Expression Regulation, Plant</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Expression des gènes</term>
<term>Phénomènes biomécaniques</term>
<term>Régulation de l'expression des gènes végétaux</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Fasciclin-like arabinogalactan proteins (FLAs) play important roles in the growth and development of roots, stems, and seeds in Arabidopsis. However, their biological functions in woody plants are largely unknown. In this work, we investigated the possible function of PtFLA6 in poplar. Quantitative real-time PCR, PtFLA6-yellow fluorescent protein (YFP) fusion protein subcellular localization, Western blotting, and immunohistochemical analyses demonstrated that the PtFLA6 gene was expressed specifically in the xylem of mature stem, and PtFLA6 protein was distributed ubiquitous in plant cells and accumulated predominantly in stem xylem fibres. Antisense expression of PtFLA6 in the aspen hybrid clone Poplar davidiana×Poplar bolleana reduced the transcripts of PtFLA6 and its homologous genes. Transgenic plants that showed a significant reduction in the transcripts of PtFLAs accumulated fewer PtFLA6 and arabinogalactan proteins than did the non-transgenic plants, leading to reduced stem flexural strength and stiffness. Further studies revealed that the altered stem biomechanics of transgenic plants could be attributed to the decreased cellulose and lignin composition in the xylem. In addition expression of some xylem-specific genes involved in cell wall biosynthesis was downregulated in these transgenic plants. All these results suggest that engineering the expression of PtFLA6 and its homologues could modulate stem mechanical properties by affecting cell wall composition in trees. </div>
</front>
</TEI>
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<DateCompleted><Year>2016</Year>
<Month>01</Month>
<Day>13</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>66</Volume>
<Issue>5</Issue>
<PubDate><Year>2015</Year>
<Month>Mar</Month>
</PubDate>
</JournalIssue>
<Title>Journal of experimental botany</Title>
<ISOAbbreviation>J Exp Bot</ISOAbbreviation>
</Journal>
<ArticleTitle>Antisense expression of the fasciclin-like arabinogalactan protein FLA6 gene in Populus inhibits expression of its homologous genes and alters stem biomechanics and cell wall composition in transgenic trees.</ArticleTitle>
<Pagination><MedlinePgn>1291-302</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/eru479</ELocationID>
<Abstract><AbstractText>Fasciclin-like arabinogalactan proteins (FLAs) play important roles in the growth and development of roots, stems, and seeds in Arabidopsis. However, their biological functions in woody plants are largely unknown. In this work, we investigated the possible function of PtFLA6 in poplar. Quantitative real-time PCR, PtFLA6-yellow fluorescent protein (YFP) fusion protein subcellular localization, Western blotting, and immunohistochemical analyses demonstrated that the PtFLA6 gene was expressed specifically in the xylem of mature stem, and PtFLA6 protein was distributed ubiquitous in plant cells and accumulated predominantly in stem xylem fibres. Antisense expression of PtFLA6 in the aspen hybrid clone Poplar davidiana×Poplar bolleana reduced the transcripts of PtFLA6 and its homologous genes. Transgenic plants that showed a significant reduction in the transcripts of PtFLAs accumulated fewer PtFLA6 and arabinogalactan proteins than did the non-transgenic plants, leading to reduced stem flexural strength and stiffness. Further studies revealed that the altered stem biomechanics of transgenic plants could be attributed to the decreased cellulose and lignin composition in the xylem. In addition expression of some xylem-specific genes involved in cell wall biosynthesis was downregulated in these transgenic plants. All these results suggest that engineering the expression of PtFLA6 and its homologues could modulate stem mechanical properties by affecting cell wall composition in trees. </AbstractText>
<CopyrightInformation>© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Haihai</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jiang</LastName>
<ForeName>Chunmei</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Cuiting</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Yang</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Lei</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>College of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gao</LastName>
<ForeName>Xiaoyan</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Hongxia</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China hxzhang@sippe.ac.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>11</Month>
<Day>26</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Exp Bot</MedlineTA>
<NlmUniqueID>9882906</NlmUniqueID>
<ISSNLinking>0022-0957</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
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<ReferenceList><Reference><Citation>Nat Protoc. 2007;2(7):1565-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17585298</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):10096-101</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12909722</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2008 Nov;56(3):351-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18644001</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2009;60(11):3133-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19433479</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2003 Sep;15(9):2020-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12953108</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2003 Oct;36(2):256-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14535889</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Dec;133(4):1911-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14645732</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biol (Stuttg). 2004 Jan-Feb;6(1):55-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15095135</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Jun 24;429(6994):873-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15215864</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Plant Biol. 2004 Aug 18;4:14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15317655</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13951-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15353603</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8255-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9223348</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Cell Dev Biol. 1997;13:171-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9442872</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2005 Nov;44(3):494-504</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16236158</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2005 Nov;17(11):3051-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16199618</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2000 Sep;12(9):1751-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11006345</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2001 Sep;47(1-2):161-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11554470</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2001 Sep;47(1-2):275-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11554476</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2001 Dec;127(4):1466-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11743090</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Tree Physiol. 2002 Apr;22(5):301-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11960754</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2010 Jan;51(1):144-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19996151</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vis Exp. 2010;(37). pii: 1745. doi: 10.3791/1745</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20224547</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vis Exp. 2010;(37). pii: 1837. doi: 10.3791/1837</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20228730</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2010;11:150</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20199690</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2010 Jun;153(2):403-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20388666</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2010 May 1;62(4):689-703</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20202165</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2011 Nov;30(11):2037-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21717184</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2011 Dec;68(6):941-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21883548</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2013 Mar;161(3):1278-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23349362</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Tree Physiol. 2013 Aug;33(8):878-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23939552</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2006 Jan;45(2):144-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16367961</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2006 May;223(6):1281-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16328545</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2006 Nov;142(3):1233-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16950861</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2007 Feb;49(4):629-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17217456</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int Rev Cytol. 2002;220:225-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12224550</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2003 Jan;15(1):19-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12509519</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Mar;131(3):1450-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12644694</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2008;179(1):94-103</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18422902</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
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Antisense expression of the fasciclin-like arabinogalactan protein FLA6 gene in Populus inhibits expression of its homologous genes and alters stem biomechanics and cell wall composition in transgenic trees.

Antisense expression of the fasciclin-like arabinogalactan protein FLA6 gene in Populus inhibits expression of its homologous genes and alters stem biomechanics and cell wall composition in transgenic trees.

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